FISH Professional Customization of Probes--A Small View and Great Application

Issuing time:2018-10-23 00:00

[perspective]

With the advancement of precision medicine, the classification of various tumor markers is becoming more and more clear, constantly updated NCCN guidelines for various diseases, CFDA to clinical laboratory self-built projectall of these make FISH's clinical application more and more widely; now FISH probe manufacturers are springing up like mushrooms, involving the clinical application of the probe species is a few, many dozens,but the import factory is more comprehensive, involves several hundred kinds.But even so, it cannot meet the growing clinical demand;because of the low incidence of some diseases, manufacturers have not produced commercial probes based on cost, which limits their application in clinical and scientific research. Beijing Bolang Sheng Technology Co, Ltd. Through various efforts, finally realized the breakthrough of customized probe, so that more patients benefit and help scientific research, here is an example of lymphoma.

[application]

Lymphoma, a complex classification, gene mutation type from copy number to rearrangement, the existing commercial probe has been unable to meet, refer to the 2016 lymphoma NCCN guidelinesto identify new applications of FISH probes in lymphoma:

Mantle cell lymphoma (MCL)

1. Copy number detection of Sox11 gene: Sox11 is a transcription factor that has been identified as a diagnostic antigen of MCL, expressed in ccnd1-negative MCL,sox11 gene deletion in MCL patients with OS shortening, in MCL accounted for more than 30%, Sox11 strong expression of OS and progression-free survival is better than weak expression.Sox11 was not expressed in other mature B-cell and T-cell lymphomas.Therefore, SOX11 can be used as a more specific marker for the diagnosis of MCL. The study also found that the cytoplasmic expression of SOX11 in MCL patients is more than that of the nucleus expressing SOX11 q21

Detection of ccnd2 translocation: the 2016 classification emphasizes the role of ccnd2 translocation (often associated with IGK / IGL translocation) in cyclin D1 / T (11;14) in the diagnosis of negative MCL, approximately 50% of cyclin D1 / T (11;14 ) Negative MCL exists CCND2 translocation.

Follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBCL)

1. FL, pediatric type, pharyngeal lymph Rings: salaverria et al. A group of FL and DLBCL, which mainly occurred in children and young people, were characterized by high expression of MUM1 / IRF4 and IRF4 gene translocation, this kind of tumor occurs in the head and neck, especially the pharyngeal lymph ring;

2. Irf4 is expressed in more than half of DLBCL, and the prognosis of those with IRF4 expression is worse than those without IRF4 expression.

Burkitt lymphoma (BL): a controversial issue in BL is whether there is a myc translocation negative BL.Studies have shown that some lymphoma with morphology similar to BL, and even phenotype and GEP are similar to BL, but there is a lack of Myc rearrangement, but there is an abnormal 11q (11q proximal acquired andtelomere loss).Compared with classical BL, these patients have complex karyotype, low expression of Myc, morphological pleomorphism and occasional follicular structure.Often show knot sex expression.Although the number of reported cases is limited, due to its clinical course similar to BL, the 2016 classification will be named "Burkitt-like lymph with 11q abnormalities" as a tentative disease.Tumor "

At present, there is no commercial probe for these mutations, but we can check the China literature, database and combined with the NCCN guide to customize the corresponding probe,to benefit more patients.

In addition, the FISH probe has a great role in the validation of samples. For example, we can do a number of studies on the relationship between a cancer and one or several genes the amount of gene chips, screening out the relevant genes, and then with custom probes, large-scale sample validation, intuitive imaging picture will make your article highmuch larger.Here is a brief introduction to our custom service.

Custom

Our company strives to improve the application of FISH in the field of diagnosis, accept custom probe service, just provide the probe reference, or detect the use of the gene name (detection of CNV,fracture rearrangement).The probe design team was personally operated by ALK probe's father, Professor Morris, Chinese American Professor Xue Liquan, and other authoritative experts.Fluorescein and other raw materials are from foreign authoritative institutions and manufacturers. Probe Features :

Probe labeling unique technology, does not contain repetitive sequence fluorescence in situ hybridization probe preparation, high specificity, brighter signal, reduce background noise,easy to observe and other characteristics;

Customized humanization, the probe type is not limited, to provide the most widely commercialized customized FISH probe, to meet a variety of scientific research and clinical needs;

The dry powder probe can be stored at room temperature, and the operation is flexible.


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